A Novato man charged with the murder of a 9-year-old girl he struck with his motorcycle while allegedly driving drunk pleaded not guilty this morning in Marin County Superior Court.Deputy Public Defender Monica Rudden told Judge Terrence Boren her client Edward John Schaefer also wished to apologize for an obscene gesture he made during his last court hearing.Schaefer is charged with murder, DUI, mayhem and vehicular manslaughter while intoxicated and with gross negligence. He is being held in the Marin County Jail under $1.5 million bail.The Marin County District Attorney’s Office said Schaefer has six prior DUI convictions.Novato police said Schaefer was driving his Harley Davidson motorcycle while intoxicated on San Marin Drive in Novato on May 27 when he struck Melodie Osheroff and her father Aaron Osheroff, 41, both of Novato. The father and daughter were crossing San Marin Drive at San Carlos Way in a crosswalk when they were struck. Novato police said Schaefer was speeding and drove through a stop sign. Authorities said his blood alcohol level was 0.16 percent, twice the level considered intoxicated.Melodie Osheroff died at Children’s Hospital in Oakland and Aaron Osheroff was taken to Santa Rosa Memorial Hospital where authorities said one of his legs was amputated. The mayhem charge against Schaefer applies to the loss of Aaron Osheroff’s leg.Schaefer suffered moderate injuries in the 8:45 p.m. crash and was in Marin General Hospital before he was transferred to the county jail.Schaefer’s preliminary hearing is scheduled for Sept. 3. It’s expected to last two days.